Fig. 2
TNF-α influences hepatocellular apoptosis in CCl4-induced acute liver injury. a TUNEL assay was performed using and b immunohistochemistry analyzed of hepatocellular apoptosis in CCl4-induced rats with anti-cleaved caspase-3 (magnification ×400). There was litter apoptosis as different doses of Enbrel were injected into the normal rats. Appropriately reduced TNF-α level would decrease hepatocellular apoptosis, and excessively reduced TNF-α level would aggravate apoptosis in CCl4-induced-acute liver injury. The arrows represented apoptotic cells. c Western-blot analyzed the expression of hepatocellular apoptosis by detecting cleaved-caspase-3 in CCl4-induced rats. Number of apoptotic hepatocytes was reduced at appropriately low level of TNF-α, while increased at high and excessive low level of TNF-α in acute liver injured rats. (*p < 0.05, **p < 0.01)