Fig. 1
Different levels of internal TNF-α influence on the CCl4-induced acute liver injured model. a Serum ALT and b AST levels were examined by Roche Diagnostic kits in Hithachi Modular P Autoanalyser. The levels of ALT and AST were significantly elevated 24 h later after subcutaneous injection of 1 ml/kg CCl4 into Sprague–Dawley rats. (ALT: from 36.75 ± 8.73 to 193.27 ± 14.26 U/L; AST: from 150.86 ± 12.34 to 253.79 ± 12.90 U/L. p < 0.01). In contrast to the CCl4 group, the ALT and AST levels were all down-regulated as Enbrel were administrated at doses of 0.25, 0.5 and 1 mg/kg, and elevated again at dose of 2, 4 and 8 mg/kg 15 min before injection of the CCl4. Data were expressed as mean ± SD of seven rats for each group. *p < 0.05, **p < 0.01. c ELISA assay of serum TNF-α was notably elevated after CCl4 administered, and gradually decreased as the doses of Enbrel were added. d Hematoxylin-eosin–stained liver sections from control and CCl4-induced rats were pretreated with different concentrations of Enbrel (scale bar, 100 μm). In the CCl4-induced acute liver injury, the hepatocellular steatosis were serious especially at 0 and 8 mg/kg Enbrel treatment, and the structures of hepatic lobule were disorder, companied with hepatocyte degeneration and inflammatory cells infiltrating. In the oil-treated rats no matter much Enbrel was pretreated, there were litter hepatocellular steatosis, and the structures of hepatic lobule were clear, with few degenerated liver cells and infiltrated inflammatory cells