Skip to main content


Fig. 1 | Cell & Bioscience

Fig. 1

From: The protective or damaging effect of Tumor necrosis factor-α in acute liver injury is concentration-dependent

Fig. 1

Different levels of internal TNF-α influence on the CCl4-induced acute liver injured model. a Serum ALT and b AST levels were examined by Roche Diagnostic kits in Hithachi Modular P Autoanalyser. The levels of ALT and AST were significantly elevated 24 h later after subcutaneous injection of 1 ml/kg CCl4 into Sprague–Dawley rats. (ALT: from 36.75 ± 8.73 to 193.27 ± 14.26 U/L; AST: from 150.86 ± 12.34 to 253.79 ± 12.90 U/L. p < 0.01). In contrast to the CCl4 group, the ALT and AST levels were all down-regulated as Enbrel were administrated at doses of 0.25, 0.5 and 1 mg/kg, and elevated again at dose of 2, 4 and 8 mg/kg 15 min before injection of the CCl4. Data were expressed as mean ± SD of seven rats for each group. *p < 0.05, **p < 0.01. c ELISA assay of serum TNF-α was notably elevated after CCl4 administered, and gradually decreased as the doses of Enbrel were added. d Hematoxylin-eosin–stained liver sections from control and CCl4-induced rats were pretreated with different concentrations of Enbrel (scale bar, 100 μm). In the CCl4-induced acute liver injury, the hepatocellular steatosis were serious especially at 0 and 8 mg/kg Enbrel treatment, and the structures of hepatic lobule were disorder, companied with hepatocyte degeneration and inflammatory cells infiltrating. In the oil-treated rats no matter much Enbrel was pretreated, there were litter hepatocellular steatosis, and the structures of hepatic lobule were clear, with few degenerated liver cells and infiltrated inflammatory cells

Back to article page