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Fig. 5 | Cell & Bioscience

Fig. 5

From: MiR-690, a Runx2-targeted miRNA, regulates osteogenic differentiation of C2C12 myogenic progenitor cells by targeting NF-kappaB p65

Fig. 5

p65 exerts its effect on Runx2-induced osteogenic differentiation partially through IL-6 upregulation. a, b The empty vector (pcDNA3.1) or p65 expression vector (pcDNA3.1-p65) was transfected into C2C12/Runx2Dox cells. The transfected cells were treated with Dox for 3 days and subjected to real-time qPCR analysis for Alp (a), OC (a) and IL-6 (b) mRNAs, respectively. Data are presented as mean ± SD (n = 3). *P < 0.05 compared with pcDNA3.1-transfected cells. Western blot was performed to assess the overexpression level of p65 (a, inset). c Real-time qPCR results of IL-6 in Dox-treated cells. Data are presented as mean ± SD (n = 3). *P < 0.05, **P < 0.01 compared with untreated cells (0 h). d The empty vector (pcDNA3.1) or IL-6 expression vector (pcDNA3.1-IL-6) was transfected into C2C12/Runx2Dox cells. The transfected cells were treated with Dox for 3 days and subjected to real-time qPCR analysis for Alp and OC mRNAs. Data are presented as mean ± SD (n = 3). *P < 0.05 compared with pcDNA3.1-transfected cells

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