Fig. 2

The effect of G-CSF on the frequency and number of long-term HSCs 1 month after TBI. Mice received vehicle or G-CSF treatment twice a day for 7 days after exposure to 0 or 4 Gy TBI as illustrated in Fig. 1a. The BM cells were collected after the mice were killed 1 month after various treatments and analyzed for long-term HSCs and short-term HSCs by flow cytometry. a Arepresentative gating strategy of LT-HSC and ST-HSC was analyzed by flow cytometry; b The frequencies of the LT-HSCs (CD34 + lineage-scal + ckit + cells) and the numbers of LT-HSCs pre mouse; c The frequencies of the ST-HSCs (CD34 + lineage-scal + ckit + cells) and the numbers of ST-HSCs pre mouse; d The frequencies of HSCs (lineage-scal + ckit + cells) and the numbers of HSCs per mouse. The data are presented as mean ± SEM, six mice/group