Fig. 3

PBA treatment caused some localization of PC_wt in GRASP55 positive vesicles. Confocal images from PC (green) (a) and GRASP55 (red) (b) stained CHO-K1 cells stably transfected with PC_wt and treated with 5 mM PBA for 48 h. Cells were stained with rabbit polyclonal anti-PC (a), and mouse monoclonal anti-GRASP55 (b). Merged images of a and b are shown in c, with co-localized green and red pixels shown in yellow color. Three independent experiments were performed. Bar in a–c 5 µm