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Fig. 3 | Cell & Bioscience

Fig. 3

From: A physical association between the human mutY homolog (hMYH) and DNA topoisomerase II-binding protein 1 (hTopBP1) regulates Chk1-induced cell cycle arrest in HEK293 cells

Fig. 3

Identification of the hMYH-interacting domain of hTopBP1. a Schematic map of the hTopBP1 deletion mutants to identify the interacting region of hTopBP1. b Co-IP and immunoblotting were performed on lysates expressing the various hTopBP1 deletion mutants. For the experiments, HEK293 cells were co-transfected with full-length c-Myc-hMYH and the various GST-hTopBP1 mutants. Co-IP was performed with an anti-c-Myc antibody, and the precipitated proteins were immunoblotted with anti-GST and anti-c-Myc antibodies

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