Fig. 1

Interaction between hTopBP1 and hMYH. a HEK293 cells were co-transfected with either GST and c-Myc-hMYH or GST-hTopBP1 and c-Myc-hMYH. Cell lysates were subjected to co-immunoprecipitation (IP) and immunoblotting. Co-IP was performed with an anti-GST antibody and protein A/G PLUS-agarose beads. Then, the precipitated proteins were immunoblotted (IB) with anti-GST and anti-c-Myc antibodies. b Co-IP was performed using an anti-c-Myc antibody in cells co-transfected with either c-Myc and GST-hTopBP1 or c-Myc-hMYH and GST-hTopBP1. The levels of the precipitated c-Myc- and GST-tagged proteins were assessed by immunoblotting. c Equal amount of purified His-hMYH was mixed with GST or GST-hTopBP1 bound glutathione Sepharose beads to pull down. Lane 1 purified His-hMYH, lane 2 and 3 pull-downs with GST or GST-hTopBP1, respectively