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Fig. 2 | Cell & Bioscience

Fig. 2

From: Hypoxic metabolism in human hematopoietic stem cells

Fig. 2

Low MP Cells are primed for hypoxic niche. a) Real-time PCR array of low MP cells compared to high MP cells shows significant upregulation of a number of hypoxia inducible genes in the low MP cells at normoxia. b) Expression of regulators of Hif-1α in low and high MP cells by RT-PCR. Note the significant downregulation of the Hif-1α destabilizing enzyme PHD2 and the upregulation of two Hif-1α stabilizing enzymes SIAH2 and VDU2 in the low MP cells. c) Survival of low MP cells under hypoxia: The viability of low MP cells under hypoxic condition, but not normoxic condition, was much higher than that of high MP cells. d) Oxygen consumption by Low MP and High MP cells at normoxia and hypoxia. Low MP cells had lower rates of oxygen consumption at normoxia compared to high MP cells, but no significant difference at hypoxia. e) Increased glycolysis rate of low MP cells in vitro measured at hypoxia. Increased glycolytic rate under hypoxia by low MP cells suggest that low MP cells could respond to hypoxia via increasing glycolysis, which indicated the low MP cells prefer glycolysis as the energy source in the physiologic hypoxic niche. f) Plots of oxygen consumption rate (OCR) as a parameter of time in low MP cells and high MP cells. g) Extracellular acidification rate (ECAR) was plotted as a parameter of time in low MP cells and high MP cells (n =3).*p < 0.05

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