Fig. 4

MED inhibits ox-LDL-induced activation of NF-κB signaling. a and b RAW264.7 cells were stimulated with ox-LDL in the absence or presence of MED for 3 h, and then western blot analysis of IκB-α and nuclear p65 was performed. Representative images of three independent experiments are shown. a, The effect of MED on ox-LDL-induced IκB-α degradation and nuclear NF-κB p65 expression. b, The effect of MED on ox-LDL-induced NF-κB DNA binding activity. RAW264.7 cells were stimulated with ox-LDL in the absence or presence of MED for 3 h, and then nuclear extracts were obtained for EMSA