Real Time PCR data analysis of miRNAs and their target mRNAs. (A) Expression profiles of 12 miRNAs in female mosquitoes at 7 days after PBS inoculation and DENV-2 inoculation. The relative expression of miRNAs were calculated against control mosquito as a calibrator using 2-ΔΔCt method and normalized to 5S rRNA. (B) Expression levels of 9 target mRNAs were detected in control and infected mosquitoes. mRNA levels relative to GAPDH were calculated. T-tests were used for the comparison of RT-qPCR data. Error bars show the standard deviation from three independent experiments with three triplicates each. Asterisks indicate the statistical significance: ***P < 0.001, **P < 0.01, *P < 0.05.