Figure 4
From: Targeted gene disruption in Xenopus laevis using CRISPR/Cas9
CRISPR/Cas9 is effective in targeting X. laevis tyrosinase. DNA sequencing data reveal the indel-inducing efficiencies of tyra-T and tyrb-T in X. laevis embryos. For all panels, the wild-type sequence is shown at the top with the target site highlighted in yellow and the PAM sequence in blue. Red dashes indicate deletions (−) and lowercase letters in red indicate insertions (+). The left two panels show data from tyra-T and tyrb-T co-injection and right two panels indicate individual injection results.