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Figure 4 | Cell & Bioscience

Figure 4

From: Development of a mouse monoclonal antibody for the detection of asymmetric dimethylarginine of Translocated in LipoSarcoma/FUsed in Sarcoma and its application in analyzing methylated TLS

Figure 4

2B12 is suitable for immunoprecipitation analysis. 2B12 was used to immunoprecipitate methylated TLS in the total cell lysate from mouse brain (A) and human brain (B). Bound methylated TLS was eluted with SDS sample buffer resolved by SDS-PAGE, and analyzed by western blotting with rabbit polyclonal anti-TLS antibody. The input lane shows 10% of the protein used in each immunoprecipitation.

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