Figure 5

ESE-1 bound to and activated GP73 promoter. (A) The empty vector (Mock) or ESE-1 or ΔESE-1 and GP73 promoter were co-transfected into Hep3B cells. The activity of GP73 promoter was measured by performing luciferase reporter assays after 36 h. (B) Similar to Hep3B cells, the activity of GP73 promoter was analyzed in Huh7 cells as (A). (C) Different doses of pCR3.1-ESE-1 plasmid and GP73 promoter were co-transfected into Hep3B cells. The activity of GP73 promoter was analyzed by completing luciferase reporter assays 36 h after transfection. (D) The sketch map of GP73 promoter is shown above. The possible ESE-1 binding sites were located in regions I (−1110/–864), III (−734/–421), and IV (−421/–79) of GP73 promoter. The results of ChIP–qPCR for ESE-1 in Hep3B cells are shown below. (E) The pCR3.1-ESE-1 plasmid and GP73 full-length promoter or deletion ESE-1 binding sites mutants were co-transfected into Hep3B cells. The activity of the promoters was analyzed by accomplishing luciferase reporter assays 36 h after transfection.