Induction of UPR target genes in H9c2 cells. H9c2 cells were left untreated or treated with (1 μM) Tg or (1 μg/ml) for 24 hours. The change in expression levels of ER stress markers was measured by qRT-PCR normalizing against GAPDH expression. The expression levels relative to the control are shown. Error bars represent mean ± S.D. from three independent experiments performed in triplicate. (*P < 0.05, two-tailed unpaired t-test compared with untreated cells).