Nuclear export of VP19C is not essential for replication of herpes simplex virus type 1

Cell & Bioscience

Table 1 Primers used for generation of recombinant VP19C-NESm/17-37BAC

Primers	Direction^a	Sequence^b
VP19C NESm	forward	CCGGCCGCAGAGCGGGCATTTGGGCGCGCACGGGCAACCAACACGATTCACGGCAC
	reverse	GTTGGTTGCCCGTGCGCGCCCAAATGCCCGCTCTGCGGCCGGCTCCAACCCG
VP19C NESm/BAC	forward	GCGCGGGTTGGAGCCGGCCGCGGAGCGGGCGTTTGGGCGCGCCCGGGCCACCAACACGATTCACGGCAAGGATGACGACGATAAGTAGGG
	reverse	CCGGGGGCGTCATGTCCTCGGTGCCGTGAATCGTGTTGGTGGCCCGGGCGCGCCCAAACGCCCGCTCCGCCAACCAATTAACCAATTCTGATTAG
BAC sequencing	reverse	TAACAACGGGGACGCTGACCG

^aDirectionality of the primer.
^bNucleotides in boldface type indicate priming sites within mutagenesis template plasmid pEP-KanS for amplification of Kan^R cassette.

ISSN: 2045-3701