|  | Sample number |
---|
Enzyme | nt position* | 9 | 10 | 13 |
---|
AfeI | 1167 | + | - | - |
FspI | 2860 | - | - | + |
DraIII (inactive) | 2925 | + | - | - |
FspI | 3940 | + | - | - |
EcoRV | 5095 | - | + | - |
BstXI | 5556 | + | - | - |
MscI (inactive) | 6352 | + | - | - |
EaeI | 6352 | + | - | - |
- Summarized in this table are distinguishable restriction enzyme cutting sites due to nucleotide substitutions introduced by long PCR amplification in three HPV58 isolates. See other details from Figure 3 legends. *The numbers are nucleotide positions with the substitutions to the reference HPV58 genome.