Skip to main content
Figure 3 | Cell & Bioscience

Figure 3

From: Genome sequencing accuracy by RCA-seq versus long PCR template cloning and sequencing in identification of human papillomavirus type 58

Figure 3

Restriction enzyme digestion distinguishes HPV58 amplicons from RCA products to their corresponding plasmids. (A) Diagrams of HPV58 amplicons from RCA samples and their corresponding plasmid clones with a new restriction enzyme cutting site. (B) Restriction enzyme digestion of RCA products and their corresponding plasmid clones from each clinical sample. RCA products and pXHW57-1 from the sample 9 were amplified using a primer pair of Pr743 and Pr1424 and digested with AfeI. RCA products and pXHW55-1 from the sample 10 were amplified using a primer pair of Pr4974 and Pr5181 and digested with EcoRV. RCA products and pXHW59-1 from the sample 13 were amplified using a primer pair of Pr2417 and Pr2960 and digested with FspI. The digested products were resolved in 1.5% agarose gel. D: digested; ND: not digested.

Back to article page

Cell & Bioscience

ISSN: 2045-3701

Contact us