Hepatic glycogen content and the expression of gene related to glycogen synthesis. (A) Hepatic glycogen content was measured from liver samples obtained in group control group (Ctrl), hypothyroid group (Hypo) and T3-treated group (T3). n = 6 in each group. (B) PAS staining of liver section from Ctrl, Hypo and T3 indicated the presence of glycogen. (C) RT-PCR analysis of GYS2 gene expression in mouse liver from Ctrl, Hypo and T3. 18s amplified in parallel served as internal reference. n = 6 ~ 8 in each group. (D) Western blotting analysis of GYS2 with α-Tubulin as an indication of equal protein loading. *P < 0.05 vs. Ctrl, ‡P < 0.05 vs. Hypo.