Figure 4

Inhibition of autophagy restored HCC cell sensitivity to chemotherapy. (A) SMMC-7721 cells (1 × 10⁴/well) were cultured in a 96-well plate with an existence of cisplatin (20 μM) and the conditioned medium collected from MSCs which were pretreated with inflammatory cytokines or not were added in SMMC-7721 culture medium for 24 hours. The occurrence of autophagy was inhibited by autophagy inhibitor-CQ (10 μM). MTT was employed to examine the proliferation of SMMC-7721 cells. (B) SMMC-7721 cells were cultured in a 6-well plate with an existence of cisplatin (20 μM) and the conditioned medium collected from MSCs which were pretreated with inflammatory cytokines or not were added in SMMC-7721 culture medium for 24 hours. The occurrence of autophagy was inhibited by autophagy inhibitor-CQ (10 μM). PI/Annexin V-FITC assay was used to measure apoptotic SMMC-7721 cells ells by flow cytometry. (C) SMMC-7721 cells (1 × 10⁴/well) were cultured in a 96-well plate with an existence of cisplatin (20 μM) and the conditioned medium collected from MSCs which were pretreated with inflammatory cytokines or not were added in SMMC-7721 culture medium for 24 hours. The occurrence of autophagy was inhibited by autophagy inhibitor-3-MA (2 mM). MTT was employed to examine the proliferation of SMMC-7721 cells. (D) SMMC-7721 cells were cultured in a 6-well plate with an existence of cisplatin (20 μM) and the conditioned medium collected from MSCs which were pretreated with inflammatory cytokines or not were added in SMMC-7721 culture medium for 24 hours. The occurrence of autophagy was inhibited by autophagy inhibitor-3-MA (2 mM). PI/Annexin V-FITC assay was used to measure apoptotic SMMC-7721 cells ells by flow cytometry. (*P < 0.05).