The degranulation of SRC-3+/+and SRC-3-/-BMMCs. (A) Identification of BMMCs. Bone marrow cells were obtained from BALB/c mice and cultured in BMMC-complete medium. After 5 weeks, cells were identified by flow cytometric analysis for FcϵRI and c-kit expression. The experiment was repeated for 3 ~ 5 times. Representative results are shown from three independent experiments. (B) BMMCs were stimulated with IgE and different concentration of DNP-HSA. Degranulation was measured by assessing hexosaminidase activity in the media or cell lysates. Values are shown as the mean + SD from three independent experiments.