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Figure 5 | Cell & Bioscience

Figure 5

From: Mitochondria directly donate their membrane to form autophagosomes during a novel mechanism of parkin-associated mitophagy

Figure 5

Co-localization of autophagosomes and cellular organelles. A. LCC9 cells were transfected with GFP-LC3 for 24 hours. Cells were incubated with either endoplasmic reticulum dye, Golgi complex-RFP, or MitoTracker-RFP and treated with 500 nM ICI to induce autophagy. Confocal microscopy was used to determine the co-localization of LC3 puncta with different cellular organelles. B. The percent of LC3-positive autophagosome co-localizing with different cellular structures was determined. n = 5-6.

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