Co-localization of autophagosomes and cellular organelles. A. LCC9 cells were transfected with GFP-LC3 for 24 hours. Cells were incubated with either endoplasmic reticulum dye, Golgi complex-RFP, or MitoTracker-RFP and treated with 500 nM ICI to induce autophagy. Confocal microscopy was used to determine the co-localization of LC3 puncta with different cellular organelles. B. The percent of LC3-positive autophagosome co-localizing with different cellular structures was determined. n = 5-6.