Mitochondria directly donate membrane material to form autophagosomes. Electron micrograph images of A. vehicle treated LCC9 breast cancer cells. B. LCC9 cells treated with 100 nM fulvestrant for 72 hours. C. LCC9 cells transfected with estrogen receptor-α shRNA. D. LCC9 cells treated with 10 μM Imatinib for 72 hours. M indicates mitochondria; Av indicates an autophagic vesicle; * indicates mitochondria-autophagosome interaction.