Figure 5

Dramatic upregulation of HAL2 by T3-treatment of premetamorphic tadpoles. Stage 54 premetamorphic tadpoles were treated with 10 nM T3 for 0-7 days and total intestinal RNA was isolated for expression analysis. Note that HAL2 expression was upregulated significantly after 1 day of treatment and the expression continued to increase dramatically during the treatment, peaking after 5 days.