Figure 5

Dramatic upregulation of HAL2 by T3-treatment of premetamorphic tadpoles. Stage 54 premetamorphic tadpoles were treated with 10 nM T3 for 0-7Â days and total intestinal RNA was isolated for expression analysis. Note that HAL2 expression was upregulated significantly after 1Â day of treatment and the expression continued to increase dramatically during the treatment, peaking after 5Â days.