Figure 7
17-DMAG upregulated serum G-CSF, inhibited radiation-induced increases in serum Flt-3 ligand, and alleviated radiation-induced WBC depletion in blood. Mice received a single oral administration of 17-DMAG (10 mg/kg) 24 h before irradiation at 8.75 Gy. Whole blood and serum were collected 30 day after irradiation. (A) Serum G-CSF (pg/ml) was detected by ELISA: * p < 0.01 vs. Veh/sham, 17-D/sham, and Veh/RI groups, error bars indicating the SEM for five independent experiments. (B) Measurement of serum Flt-3 ligand (pg/ml) by ELISA: * p < 0.001 vs. Veh/sham, 17-D/sham, and 17-D/RI groups; ** p < 0.001 vs. Veh/sham, 17-D/sham and Veh/RI, error bars indicating the SEM for six independent experiments. (C) WBC counts (cells/ml): * p < 0.001 vs. Veh/sham, 17-D/sham, 17-D/RI groups; ** p < 0.001 vs. Veh/sham, 17-D/sham and Veh/RI groups, error bars also indicating the SEM for six independent experiments. Veh: vehicle; 17-D: 17-DMAG; RI: radiation injury.