Figure 1

Inhibition of autophagy accelerated ischemia-induced liver injury. (A) The rats were treated as the indicated ischemic time, and then their liver fractions were analyzed by immunoblot assay. Semiquantitative densitometry analysis (versus GAPDH) of LC3 II was performed for each sample. Data were shown as mean ± SEM (n = 3). (B) Four groups of rats were treated as indicated. After 90 min of ischemia treatment, the protein levels of LC3 and p62 in their liver sections were analyzed by immunoblot assay. Data were shown as mean ± SEM (n = 3). (C) The liver samples were processed for EM. And representative electron micrographs were shown. The photos at the bottom panel were high magnification of electron micrographs. Arrows denote autophagic vacuoles (N: nucleus; M: mitochondria; Bar: 1 μm). (D) The number of autophagosome per 100 μm² in the electron micrographs was determined. Data were shown as mean ± SEM (n = 6; *: p < 0.05; **: p < 0.01). (E and F) The serum ALT (E) and AST (F) levels of rats from the indicated groups were detected. Data were shown as mean ± SEM (n = 6; *: p < 0.05; **: p < 0.01). (G) Liver samples of the indicated groups were processed for H&E. Representative images were shown with an original magnification × 200.