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Table 3 Colony PCR mix in Golden Gate cloning round 1

From: Generation of gene disruptions by transcription activator-like effector nucleases (TALENs) in Xenopus tropicalis embryos

Components PCR Mix (μL)
5 × GoTaq Buffer 5
25 mM MgCl2 2.5
10 mM dNTP Mix (Fermentas) 0.5
Primer Fw (10 mM) 0.5
Primer Re (10 mM) 0.5
Colony suspension 5
GoTaq DNA polymerase (Promega) 0.5
dH2O 10.5
Total 25