Skip to main content

Table 2 Ligation program in Golden Gate cloning round 1

From: Generation of gene disruptions by transcription activator-like effector nucleases (TALENs) in Xenopus tropicalis embryos

Cycle number Temperature (°C) Time (min)
1-15 37 5
  16 10
16 16 15
17 50 10
18 80 5