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Table 1 Ligation mix in Golden Gate cloning round 1

From: Generation of gene disruptions by transcription activator-like effector nucleases (TALENs) in Xenopus tropicalis embryos

Components pFUS_A Mix (μL) pFUS_B Mix (μL)
Buffer 4 (NEB) 1 1
T4 Ligase (NEB) 0.8 0.8
BsaI (NEB) 0.8 0.8
RVD vectors (150 ng/μL) 0.6 × 10 0.6 × #N-1
pFUS_A/B vectors (150 ng/μL) 1 1
ATP (25 mM) 0.4 0.4
dH2O 0 6-(0.6 × #N-1)
Total 10 10