Table 2 The genetic characterization of the transcriptional modulator MazF , a chromosomal cell death factor from potential probiotic strains and some major food-borne pathogens used in this study
Organism | Type of organisms | Strains | Protein accession (GenBank) | Gene annotation |
|---|---|---|---|---|
E. coli | Pathogen | EDL933 | NP_289336.1 | toxin ChpA |
| Â | Pathogen | Sakai | NP_311669.1 | Â |
| Â | Antibiotic resistance | B185 | ZP_06660634.1* | Â |
Enterobacter sp. | pathogen | 638 | ABP60743.1* | transcriptional modulator of MazE/toxin, MazF |
Shigella dysenteriae | pathogen | Sd197 | YP_405833.1* | toxin ChpB |
Citrobacter rodentium | pathogen | ICC168 | YP_003366767.1* | Toxin component of the ChpB-ChpS toxin-antitoxin system |
Vibrio vulnificus | pathogen | CECT4999=R99 | YP_001393091.1* | growth inhibitor |
Listeria welshimeri serovar 6b str. | pathogen | SLCC5334 | YP_849071.1 | PemK family transcriptional regulator |
| Â | Â | FSL J1-208 | ZP_05296226.1* | PemK family transcriptional regulator |
Listeria monocytogenes | Â | SLCC3954 | YP_003464028.1 | transcriptional regulator, PemK family |
| Â | Â | FSL S4-120 | ZP_07870171.1 | toxin-antitoxin system, antitoxin component, MazF family |
Listeria seeligeri serovar 1/2b str. | Â | Â | Â | Â |
Listeria marthii | Â | Â | Â | Â |
Listeria grayi | pathogen | DSM 20601 | ZP_07054243.1* | MazF family toxin-antitoxin system protein |
Clostridium difficile | pathogen | 630 | YP_001089981.1* | putative regulator of cell growth |
| Â | Â | QCD-66c26 | ZP_05273557.1 | putative regulator of cell growth |
| Â | Â | CIP 107932 | ZP_05323891.1 | putative regulator of cell growth |
Klebsiella variicola | pathogen | At-22 | YP_003438577.1* | transcriptional modulator of MazE/toxin, MazF pemK; protein PemK |
| Â | Â | 1_1_55 | ZP_06548086.1 | Â |
Salmonella enterica subsp. arizonae serovar | pathogen | RSK2980 | YP_001573441.1* | hypothetical protein SARI_04525 |
Campylobacter showae | pathogen | RM3277 | ZP_05364729.1 | addiction module antitoxin, RelB/DinJ family |
Campylobacter concisus | pathogen | 13826 | YP_001466677.1 | addiction module antitoxin |
Campylobacter jejuni subsp. | pathogen | IA3902 | ADC28395.1 | prevent-host-death family protein |
Campylobacter upsaliensis | pathogen | JV21 | ZP_07894578.1* | ChpA/MazF transcriptional modulator |
Shigella flexneri 2a str. | pathogen | 301 | NP_709188.1* | PemK protein |
Shigella flexneri 5 str. | Â | 8401 | YP_690766.1 | growth inhibitor, PemK-like, autoregulated |
Cronobacter sakazakii | pathogen | ATCC BAA-894 | YP_001436394.1 | bifunctional antitoxin/transcriptional repressor RelB |
Lactobacillus coleohominis | probiotic | 101-4-CHN | ZP_05553821.1* | regulatory protein |
Bacillus megaterium | probiotic | QM B1551 | YP_003560763.1* | endoribonuclease EndoA |
| Â | Â | DSM 319 | YP_003595503.1 | Â |
Bacillus thuringiensis serovar israelensis | probiotic | ATCC 35646 | ZP_00740711.1* | MazF protein |
| Â | Â | KBAB4 | YP_001643128.1 | MazF protein |
Bacillus weihenstephanensis | Â | AH1134 | ZP_03233130.1 | PemK family |
Bacillus cereus | Â | Â | Â | Â |
Coprobacillus sp. | probiotic | 29_1 | ZP_08009764.1* | PemK family transcriptional regulator |
Enterococcus faecalis | probiotic | V583 | NP_814592.1 | PemK family transcriptional regulator |
| Â | Â | TX0102 | EFQ11602.1* | Â |
| Â | Â | Â | Â | Toxin-antitoxin system, toxin compoment, MazF |
| Â | Â | TX2134 | ZP_07557645.1* | Â |
| Â | Â | Â | Â | Toxin-antitoxin system, toxin compoment, MazF |
| Â | Â | HH22 | ZP_03985009.1 | Â |
| Â | Â | Â | Â | PemK family transcriptional regulator |
Lactobacillus plantarum | probiotic | WCFS1 | NP_786238.1* | cell growth regulatory protein |
Lactobacillus rhamnosus | probiotic | LMS2-1 | ZP_04441477.1* | cell growth regulatory protein |
| Â | Â | Lc 705 | YP_003175134.1 | transcriptional modulator of MazE/toxin, MazF |
Lactobacillus johnsonii | probiotic | ATCC 33200 | ZP_04007131.1* | PemK family growth inhibitor |
Lactobacillus crispatus | probiotics | CTV-05 | ZP_07789240.1* | ppGpp-regulated growth inhibitor |
Lactobacillus gasseri | probiotic | ATCC 33323 | YP_815460.1* | toxin-antitoxin system, toxin component, MazF family |
| Â | Â | 224-1 | ZP_06262236.1 | Â |
| Â | Â | MV-22 | ZP_07711551.1 | Â |
Lactobacillus casei | probiotic | BL23 | YP_001986080.1 | Cell growth regulatory protein |
Lactobacillus amylovorus | probiotic | GRL1118 | YP_005854914.1* | transcriptional modulator of MazE/toxin MazF |
Vibrio cholerae | probiotic | 1587 | ZP_01950611.1* | transcriptional modulator of MazE/toxin, MazF |
| Â | Â | NCTC 8457 | ZP_01969676.1 | Â |
Pediococcus pentosaceus | probiotic | ATCC 25745 | YP_805020.1* | toxin-antitoxin addiction module toxin component MazF (an endoRNAse) |
Bacillus amyloliquefaciens | probiotic | Y2 | YP_006327269.1* | Endoribonuclease |
Bacillus pumilus | probiotic | SAFR-032 | YP_001485694.1* | PemK family growth inhibitor endoribonuclease EndoA |
| Â | Â | ATCC 7061 | ZP_03054587.1 | Â |
Lactobacillus sakei subsp. sakai | probiotic | Sakei 23K | YP_396224.1 | DNA-binding protein PemK family |
Leuconostoc mesenteroides subsp. mesenteroides | probiotic | ATCC 8293 | YP_819271.1* | toxin-antitoxin addiction module toxin component MazF |
| Â | Â | ATCC 19254 | ZP_03913232.1 | PemK family growth inhibitor |
Leuconostoc mesenteroides subsp. cremoris | Â | Â | Â | Â |
Enterococcus faecalis | probiotic | TX0102 | EFQ11602.1* | toxin-antitoxin system, toxin component, MazF family |
| Â | Â | TX0031 | EFT96737.1 | Â |
Bifidobacterium animalis subsp. lactis | probiotic | HN019 | ZP_02964075.1* | transcriptional modulator of MazE/toxin, MazF |
Bifidobacterium bifidum | probiotic | NCIMB 41171 | ZP_03645723.1 | RelB antitoxin |
Bifidobacterium longum subsp. longum | probiotic | JDM301 | YP_003660624.1* | RelB antitoxin |
| Â | Â | DSM 20213 | ZP_06596513.1 | toxin-antitoxin system protein |
Bifidobacterium breve | Â | Â | Â | Â |
Bifidobacterium bifidum | probiotic | S17 | YP_003938101.1 | hypothetical protein with RelB antitoxin domain |
Lactobacillus casei | Probiotic | ATCC 334 | YP_807723.1 | toxin-antitoxin addiction module toxin component MazF (an endoRNAse) |
Lactobacillus casei | Probiotic | BL23 | YP_001988635.1 | Growth inhibitor |
Lactobacillus paracasei subsp. paracasei | Probiotic | ATCC 25302 | ZP_03963082.1* | PemK family transcriptional regulator |
Lactobacillus casei str. | probiotic | Zhang | YP_003789562.1 | toxin-antitoxin addiction module toxin component MazF |
Lachnospiraceae bacterium | probiotic | 3_1_57FAA_CT1 | ZP_08609193.1* | hypothetical protein HMPREF0994_05199 |
Leuconostoc citreum | Probiotic | KM20 | YP_001727503.1* | growth inhibitor |
Lactobacillus reuteri | probiotic | DSM 20016 | YP_001270863.1* | transcriptional modulator of MazE/toxin, MazF |
| Â | Â | JCM 1112 | YP_001841242.1 | hypothetical protein LAR_0246 |
| Â | Â | MM2-3 | ZP_03847756.1 | PemK family growth inhibitor |
Streptococcus sp. oral taxon 071 str. | probiotic | 73H25AP | ZP_07458552.1* | ChpA/MazF transcriptional modulator |
Bacillus selenitireducens | probiotic | MLS10 | YP_003700699.1* | transcriptional modulator of MazE/toxin, MazF |
Bacillus halodurans | probiotic | C-125 | NP_244588.1* | ppGpp-regulated growth inhibitor (ChpA/MazF) |
Enterococcus faecium | probiotic | DO | ZP_05714797.1* | PemK family protein |
| Â | Â | TX0133a04 | ZP_07845579.1 | toxin component, MazF family |
| Â | Â | TX0133C | ZP_07850444.1 | toxin component, MazF family |