Figure 3

pcDNA3-CRT/LT generates the most LT-specific (aa 19–27) CD8⁺T cells. C57BL/6 mice (5 mice/group) were immunized with DNA vaccines by gene gun in the same schedule as Figure1A. Pooled splenocytes from mice vaccinated with pcDNA3 vector (control), pcDNA3-LT, and pcDNA3-CRT/LT were collected and cultured in vitro with either no peptide or amino acid 19–27 then stained for intracellular IFN-gamma and CD8⁺ T cell surface marker. (A) Representative flow cytometry dot plot of LT-specific CD8⁺ T cell activation after stimulation with amino acid sequence 19–27. (B) Representative bar graph of flow cytometric data.