Wnt/β-catenin signaling activation induces the level of miR-181. (A) qRT-PCR analysis of all four mature miR-181s in HuH7 and HuH7 WNT10B R8 cells (left panel) and in HuH7 cells cultured with normal media or conditioned media from HuH7 WNT10B R8 for 3 days (right panel). (B) qRT-PCR of miR-181s in HuH1 and HuH7 cells incubated with LiCl or NaCl for 3 days. (C) qRT-PCR analysis of all four mature miR-181s in HuH7 cells and HuH1 cells with transient transfection of β-catenin, wild-type Tcf4 (wt-Tcf4), dominant negative Tcf4 (dn-Tcf4) or control for three days. The experiments were performed in triplicates and results were shown as mean ± standard deviation. * refers to p < 0.05.