Effects of fatty acids on LEF2 cells. A) Appearance of cells 24 hrs following exposure to oleic acid (OA, 1 mM), palmitic acid (PA, 1 mM), both (OA+PA, 3 mM at 2:1 ratio) or vehicle control. Magnification 10×. Inserts represent higher magnification views showing cytoplasmic vesicles. B) Assessment of cell viability (Trypan blue exclusion), proliferation (MTT), and ATP levels following 6 hours of fatty acids exposure. C) Western blot analyses of cell lysates from each of the four treatments blotted for the indicated proteins. α-tubulin serves as a loading control. For graphs, error bars represent SEM. *, p < 0.05; ◆ indicates p < 0.01 compared to all other groups; O indicates p < 0.05 compared to all other groups.