Table 1 Oligonucleotide primers used in this study.
From: Transient knockdown and overexpression reveal a developmental role for the zebrafish enosf1b gene
Primer name | Primer sequence |
|---|---|
Enosf1b full length forward | ATGCTGGCGATCAAAATCATA |
Enosf1b full length reverse | CTGCTGTTTCTCAATGGCTCT |
Enosf1b XhoI Kozak forward | ATACTCGAGGACACCATGCTGGCGATCAAA |
Enosf1b SacII reverse | CTCGAGCCGCGGATTTTTCTGCTGTTTCTC |
Exon 10 flanking forward | GCCCTTGTGGAAGCTACTTG |
Exon 10 flanking reverse | GTGGGGCTTTTGAAGTGTTC |