Co-localization of LAMP-1 in NIH/3T3. NIH/3T3 cells show a similar pattern of vacuole formation with treatment of chloroquine. Phase contrast and corresponding LAMP-1 co-localization of 25 μg/ml chloroquine-treated cells (C, D) show vacuolation of LAMP-1, when compared with control (A, B). The arrows in C and D note that the borders of the vacuoles correspond with LAMP-1 protein. Bar size = 25 microns.