Phosphorylation of LSD1 by PLK1 promotes its chromatin release during mitosis
- Bin Peng†1, 2,
- Ruifeng Shi†1,
- Weiwei Jiang†1,
- Yue-He Ding3,
- Meng-Qiu Dong3,
- Wei-Guo Zhu2 and
- Xingzhi Xu1, 2Email author
© The Author(s) 2017
Received: 14 March 2017
Accepted: 17 March 2017
Published: 23 March 2017
Lysine-specific histone demethylase 1 (LSD1) modulates chromatin status through demethylation of H3K4 and H3K9. It has been demonstrated that LSD1 is hyperphosphorylated and dissociates from chromatin during mitosis. However, the molecular mechanism of LSD1 detachment is unknown.
In this report, we found that polo-like kinase 1 (PLK1) directly interacted with LSD1 and phosphorylated LSD1 at Ser-126 . Nocodazole-induced metaphase arrest promoted release of LSD1 from chromatin, and the phosphorylation-defective mutant LSD1 (S126A) failed to dissociate from chromatin upon nocodazole treatment.
Taken together, our findings demonstrate that phosphorylation of LSD1 at Ser-126 by PLK1 promotes its release from chromatin during mitosis.
KeywordsPLK1 Lysine-specific demethylase LSD1 Phosphorylation Mitosis Chromatin release
Histone post-translational modifications (PTMs) serve as major regulatory mechanisms for chromatin structure and function . While the presence of methylation and acetylation  and phosphorylation  on histones was described about half a century ago, many more PTMs have been demonstrated to occur on histones . Given that chromatin dynamics plays an essential role both in maintenance of cellular homeostasis and in cellular stress responses, histone PTMs have been implied in most, if not all physiological and pathological processes. However, the biological significance and the molecular mechanism of these PTMs, particularly the crosstalk and joint effects of different PTMs on the same molecule, are largely elusive, and deciphering the regulatory networks of the protein machineries that incorporate (write), remove (erase), and bind (read) histone PTMs is of high interest.
Lysine-specific demethylase 1 (LSD1), a flavin adenine dinucleotide (FAD)-dependent amine oxidase, was the first identified demethylase (eraser) for lysine methylation of histones and non-histone proteins . It specifically removes methyl groups via a redox process of mono- or di-methylated histone H3 lysine4 (H3K4)  and H3 lysine 9 (H3K9) . LSD1 in the CoREST-HDAC containing repressor complexes, functions as a corepressor by mediating demethylation of H3K4me . On the other hand, LSD1 is recruited to the promoter regions of androgen receptor (AR) target genes and demethylates H3K9me, co-activating AR-dependent transcription . Misregulated expression of LSD1 has been reported in several cancer types [5, 8].
Our lab reported previously that LSD1 is hyperphosphorylated in response to nocodazole-induced metaphase arrest . It was demonstrated recently that LSD1 dissociates from chromatin during mitosis . We thus sought to uncover a potential link between LSD1 and mitotic kinases. Polo-like kinase 1 (PLK1), a major mitotic serine/threonine protein kinase, regulates several events throughout M phase of the cell cycle, from mitotic entry to mitotic exit and cytokinesis . Its functions are executed by binding and phosphorylating proteins through its polo-box domain and kinase domain, respectively. In this report, we found that PLK1 interacts with and phosphorylates LSD1 at Ser126 and this phosphorylation promotes LSD1 release from chromatin during mitosis.
Results and discussion
LSD1 directly interacts with PLK1
PLK1 phosphorylates LSD1 at Ser-126
Phosphorylation of LSD1 by PLK1 promotes its release from chromatin during mitosis
Histone methylation at specific lysine residues plays a critical role in regulating chromatin structure and gene expression and subsequent cellular activities. Fine control of the balance of histone methyltransferase/demethylase activities is essential for cell cycle progression and maintenance of genome integrity . Tipping the balance toward either direction could be detrimental. For example, LSD1 serves for demethylation of H3K4 and H3K9 , its gene deletion was reported in pancreatic ductal adenocarcinoma (7/109 = 6.4%) , while its gene amplification was found in neuroendocrine prostate cancer (6/107 = 5.6%) (http://www.cbioportal.org/) and sarcoma (6/207 = 2.9%) . LSD1 RNA expression levels vary a lot in different cancer types (http://www.cbioportal.org/). These findings imply that a delicate control of LSD1 expression levels, subcellular localization, and enzymatic activity may be a requirement for genome stability. Whole-genome mapping demonstrated that LSD1 genomic regions overlaps with H3K4me2 genomic regions and both are enriched on the promoters of highly expressed genes in ES cells , while H3K9me3 levels peak at the pericentromeric regions during mitosis [13–15]. To reduce general transcriptional activity to a minimum and maintain high H3K9me3 levels at the pericentromeric regions during mitosis, histone demethylase activities on chromatin should be reduced.
Our study has uncovered a novel regulatory mechanism for controlling histone demethylase activity, in which PLK1-mediated phosphorylation of LSD1 during mitosis expels it from chromatin.
Cell cultures, reagents, antibodies
HeLa and HEK293T cells were grown in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum at a 37 °C incubator with 5% CO2.
Nocodazole (M1404, a final concentration of 340 nM was used throughout this research) and Thymidine (T1895, a final concentration of 2 mM was used) were purchased from Sigma. BI2536, a PLK1 inhibitor (S1109, a final concentration of 1 μM was used), was purchased from Selleck.
Rabbit polyclonal antibodies used for immunoblotting and immunoprecipitation in this study including anti-HA (A190–208A), anti-LSD1 (A300–215A), anti-CoREST (A300–130A), anti-GAPDH (A300–643A) and anti-H3 Ser10 (A301–844A) were from Bethyl Laboratories. Rabbit polyclonal anti-H3 antibody (#9715) was from Cell Signaling Technology. Mouse monoclonal antibody against GST (A00865) was from GenScript Corporation. Mouse monoclonal anti-FLAG M2 (F1804) was from Sigma. Mouse monoclonal anti-HIS (D291-3) was from MBL Biotech.
Human cDNA clones encoding full length and deletion mutants of LSD1, PLK1 were subcloned into pcDNA3.0 with three copies of HA or FLAG epitope at its N-terminus for expression in mammalian cells or into pET28(A) or pGEX-4T-1 for producing HIS or GST recombinant fusion protein in E. coli. Point mutants (LSD1(S126A), LSD1(S131A), and LSD1(2A)) were generated using the QuikChange mutagenesis kit (SBS Genetech Co).
Immunoblotting and immunoprecipitation
GST-pulldown and in vitro kinase assay
Bacterially-purified GST fusions (1 μg) were incubated with bacterially produced HIS tagged fusions (1 μg) in 500 μl of NETN buffer at 4 °C overnight. Glutathione-Sepharose beads (20 μl per pulldown) were added and incubated for 1 h before extensively washing with NETN buffer.
For in vitro kinase assays [12, 22], bacterially-purified HIS tagged PLK1 and LSD1 were incubated at kinase buffer containing 50 mM HEPES (pH 7.4), 10 mM MgCl2, 1 mM DTT, 1 mM Na3VO4, 10 μM coldATP and 5 μCi [γ-32P]-ATP at 30 °C for 30 min.
Identification of phosphorylation sites of LSD1 by PLK1 using mass spectrometry
Phosphorylation site mapping by mass spectrometry was performed as described previously . Briefly, in vitro phosphorylated proteins were precipitated with TCA and resuspended in a buffer containing 8 M urea, 100 mM Tris, pH8.5. After reduction and alkylation, the sample was digested by Trypsin overnight at 37 °C. The peptides were analyzed on an Easy-nLC 1000 UPLC (Thermo Fisher Scientific) coupled to a Q exactive mass spectrometer (Thermo Fisher Scientific). Peptides were loaded on a pre-column (75 μm ID and packed with 8 cm ODS-AQ 12 nM S-10 mm (YMC Co., Ltd)) and separated on an analytical column (75 μm ID and packed with 11 cm Luna 3 μm 100Å resin (Phenomenex)) with an acetonitrile gradient from 0 to 30% in 55 min and 30–80% in another 10 min at a flow rate of 300 nl/min. Spectra were acquired in a data-dependent mode: the 10 most intense ions except charge 1+ or unassigned from each full scan (Resolution 70,000) were isolated for HCD MS2 (Resolution 17,500) at NEC 27 with a dynamic exclusion time of 60 s. For peptide identification, the MS2 spectra were searched against an artificial database (including GST-LSD1 and HIS-PLK1 sequences in a C. elegans WS217 database) using Prolucid . Search results were filtered using DTASelect 2.0  with 7 p.p.m. mass accuracy for precursor mass and a 5% FDR cutoff. The phosphorylation spectra presented in the figures were annotated using pLabel .
Chromatin fractionation and cell synchronization
corepressor for element silencing transcription factor
cyclin-dependent kinase 1
casein kinase II
histone H3 lysine 4
histone H3 lysine 9
lysine-specific demethylase 1
polo-like kinase 1
XX, WGZ, MQD, and BP conceived the project and analyzed the data; BP, RS, WJ, and YHD performed the experiment; XX and BP wrote the manuscript. All authors have agreed to make all data and material in the manuscript available. All authors have read and approved the final manuscript.
We thank other members of the Xu laboratory for help.
The authors declare that they have no competing interests.
This work was supported by the National Natural Science Foundation of China (NSFC) Grants 31530016 and 31461143012, the 973 projects 2013CB911002 and 2015CB910601 to X.X.
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