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Fig. 8 | Cell & Bioscience

Fig. 8

From: Timely expression of PGAM5 and its cleavage control mitochondrial homeostasis during neurite re-growth after traumatic brain injury

Fig. 8

FCCP induces PGAM5 cleavage and promotes motor function recovery of CCI mice A Immunoblots of PGAM5 in neuro2a cells treated with DMSO (ctrl), 0.1 μM or 1.0 μM FCCP for 24 h. FL: full-length. B The percentage of cleaved PGAM5 was quantified by cleaved PGAM5/total PGAM5. Data are presented as mean ± SEM (n = 6). ** p < 0.01, one‑way ANOVA with Tukey’s multiple comparisons. C Mitochondria were visualized by MitoBright LT Deep Red in neuro2a cells. Scale bar, 10 μm. D Quantification of total intensity of MitoBright in individual neuro2a cells. Dashed lines indicate the medium and dotted lines indicate the 25th and the 75th percentiles (n = 140–159 cells/group). * p < 0.05, *** p < 0.001, one‑way ANOVA with Dunnett’s multiple comparisons. E Immunoblot of PGAM5 and TFAM in mice brain on dpi 7. Veh, vehicle control. F, G Quantification of PGAM5 and TFAM in (E). Data are presented as mean ± SEM (n = 7). * p < 0.05, one‑way ANOVA with Tukey’s multiple comparisons. (H) Timeline of the experimental design for rotarod test and grid test. C57BL/6 J mice were intranasally administrated 0.1 mg/kg FCCP, 1.0 mg/kg FCCP or vehicle (DMSO) at 6 h after CCI on 0 dpi. Rotarod test was performed on 1–4 dpi. Grid test was performed on -1, 1, 3 and 6 dpi. I Quantification of latency to fall of mice on 1–4 dpi. Data are presented as mean ± SEM (n = 6). * p < 0.05, ** p < 0.01, one‑way ANOVA with Tukey’s multiple comparisons. J Quantification of foot faults of CCI mice on -1, 1, 3 and 6 dpi. Data are presented as mean ± SEM (n = 7). ** p < 0.01, *** p < 0.001, **** p < 0.0001, one‑way ANOVA with Tukey’s multiple comparisons

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