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Fig. 6 | Cell & Bioscience

Fig. 6

From: Timely expression of PGAM5 and its cleavage control mitochondrial homeostasis during neurite re-growth after traumatic brain injury

Fig. 6

TBI induces loss of ΔΨm and reduces mitochondrial PGAM5 A Mitochondrial membrane potential of hippocampal neurons was accessed by TMRM staining. White dashed lines indicate the borders of the injured gap. Scale bar, 50 µm. B Quantification of mean intensity of TMRM in individual hippocampal neurons. Dashed lines indicate the medium and dotted lines indicate the 25th and the 75th percentiles (n = 115–127 neurons/group). **** p < 0.0001, unpaired t-test. C Immunofluorescence of PGAM5 (green) and mitochondrial outer membrane protein TOM20 (red) in hippocampal neurons. Scale bar, 20 µm (TOM20 staining) and 10 µm (merged images). D The percentage of mitochondrial PGAM5 was quantified by PGAM5+TOM20+ area/PGAM5+ area. E Quantification of TOM20 intensity in hippocampal neurons. Dashed lines indicate the medium and dotted lines indicate the 25th and the 75th percentiles (n = 110 neurons/group). * p < 0.05, *** p < 0.001, unpaired t-test

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