Fig. 4

Cleaved PGAM5 enhances neurite re-growth in injured cortical neurons A Schematic model of PGAM5, PGAM5(Δ2-24), hPARL(AAA) and hPARL(DDD) constructs. TD, transmembrane domain. B Images of injured cortical neurons overexpressing PGAM5, PGAM5(Δ2-24), wild-type hPARL, hPARL(AAA) and hPARL(DDD) on DIV9. Cortical neurons were visualized by co-transfection of EGFP vector. Yellow dashed lines indicate the borders of injured gap. Scale bar, 100 μm. C Quantification of the length of re-growing neurites in (B). Dashed lines indicate the medium and dotted lines indicate the 25th and the 75th percentiles (n = 209–238 cells/group). *** p < 0.001, **** p < 0.0001, one‑way ANOVA with Dunnett’s multiple comparisons. D Images of injured cortical neurons transiently transfected with shlacZ, shPgam5 or shParl vectors on DIV9. Cortical neurons were visualized by co-transfection of EGFP vector. Yellow dashed lines indicate the borders of injured gap. Scale bar, 100 μm. E Quantification of the length of re-growing neurites in (D). Dashed lines indicate the medium and dotted lines indicate the 25th and the 75th percentiles (n = 220–273 cells/group). **** p < 0.0001, one‑way ANOVA with Dunnett’s multiple comparisons