Establishing extended pluripotent stem cells from human urine cells

Cell & Bioscience

Table 1 OCM175 medium components (50 mL)

Component	Volume added	Storage Concentration
bFGF2	250 μL	20 μg/mL
IGF2	8 μL	0.5 mg/mL
TGFβ1	2.5 μL	10 μg/mL
Insulin	100 μL	10 mg/mL
L-Selenium-Methylselenocysteine	5 μL	100 μg/mL
Transferrin	2 μL	50 mg/mL
GSH	200 μL	50 mg/mL
VC	20 μL	70 mg/mL
NEAA	500 μL	100 ×
EAA	250 μL	200 ×
GlutaMAX-I	100 μL	500 ×
Heparin	10 μL	10 mg/mL
HSA	200 μL	100 mg/ml
B27	1 mL	50 ×
NaHCO₃	800 μL	0.25 M
KO-DMEM	25 mL
DMEM/F12	20.6 mL
B27	1 mL
Osmotic pressure (mosm/kgH₂O)	~ 311

ISSN: 2045-3701