Fig. 4

EVs derived from transfected cells affect migration and invasiveness of NB cells. A. qPCR analysis of miR-210-3p levels in SK-N-AS and SK-N-DZ cells and derived EVs transfected with miR-210-3p mimic and cultured in normoxic condition. B. qPCR analysis of miR-210-3p levels in NB cells and EVs transfected with miR-210-3p inhibitor and cultured in hypoxic condition. Data are represented as log2(2-ΔΔCt). Values are expressed as mean ± SEM. ****p < 0.0001 vs miR-NC. C-D. Representative images of wound healing assay performed on cells treated with EVs from transfected NB cells with miR-210-3p mimic and miR-210-3p inhibitor; images were acquired at the time of EVs addition (t0) and 48 h after treatment (t48). Magnification 10X. Scale bar, 100 µm. Wound closure areas and cell velocity (µm/h) were quantified with Image J. Values are expressed as mean ± SEM from 3 independent experiments. E–F. Quantification of cells invaded toward the Matrigel layer (average of 5 picture fields at 10 × magnification) via Image J. nEVs-210mimic data are normalized on the averaged value for nEVs, hEVs-210inhibitor are similarly normalized on hEVs-NC. Values are mean ± SEM