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Fig. 1 | Cell & Bioscience

Fig. 1

From: miR-210-3p enriched extracellular vesicles from hypoxic neuroblastoma cells stimulate migration and invasion of target cells

Fig. 1

Isolation and characterization of EVs from NB cell culture conditioned media. A. Schematic representation of the experimental flow. Arrows indicate media changes and collection of CM for EVs isolation. B. Relative mRNA expression of the hypoxia markers CA9 and VEGF measured by qPCR in SK-N-AS and SK-N-DZ cells cultured in normoxia (grey box), hypoxia (red box), and reoxygenation (blue box) conditions. *p < 0.05 and ****p < 0.0001 vs normoxia. C. Representative TEM images show the morphology of EVs. Insert indicates immunogold labeled EVs with anti CD81 antibody. D. Validation of EVs marker expression by western blot analysis. E. EVs concentration normalized by number of SK-N-AS and SK-N-DZ cells cultured in normoxia (grey box), hypoxia (red box), and reoxygenation (blue box) conditions as quantified by qNano. *p < 0.05 vs normoxia F. Heatmap depicting the background-corrected signals of 18 out the 39 markers expressed on the surface of NB-EVs. G. Bar graph showing enrichment of the top10 biological signaling pathway components of the upregulated proteins in common between the two cell lines

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