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Fig. 5 | Cell & Bioscience

Fig. 5

From: Hinokitiol-iron complex is a ferroptosis inducer to inhibit triple-negative breast tumor growth

Fig. 5

Effects of Fe(hino)3 on TNBCs-induced tumor growth. A 4T-1 cells were orthotopically injected into the mammary pad of female Balb/c mice. A schematic graph to use Fe(hino)3 (2 mg/kg, i.p.) in a 4T-1 cell orthotopic tumor model. B Tumor size was recorded on the indicated days. C Representative isolated-tumor images from each group on day 25 after treatment with Fe(hino)3 for 18 days. D The weight of isolated tumors on day 25. E The content of 4-HNE and expression of Ki67, detected by immunohistochemistry, and the iron level, detected using DAB-enhanced Perl’s staining in tumors. F Upper panel: the tumors metastasized to the lungs, revealed by H&E staining and marked with *. Lower panel: the DAB-enhanced Perl’s iron staining and the iron enrichment marked with arrows. G, H Representative isolated-tumors and tumor weight from each group on day 21 after two shots with Fe(hino)3 through intratumor injection. *, p < 0.05; ***, p < 0.001

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