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Fig. 3 | Cell & Bioscience

Fig. 3

From: Hinokitiol-iron complex is a ferroptosis inducer to inhibit triple-negative breast tumor growth

Fig. 3

Fe(hino)3 triggers the ferroptosis of TNBCs. A Cell viability of MDA-MB-231 cells treated with 5 µM Fe(hino)3 following pretreatment with different inhibitors of cell death for 24 h. Trolox (200 µM), Fer-1 (5 µM), z-VDA-FMK (25 µM), VX765 (10 µM), NFA (2 µM). B Malondialdehyde (MDA) content in MDA-MB-231 cells treated with Fe(hino)3 for 24 h, measured with MDA detection kit. C–D Lipid ROS (C) and general ROS levels (D) detected by flow cytometry with BODIPY-C11 and H2-DCFDA, respectively, in MDA-MB-231 cells following treatment with Hino (15 µM) or Fe(hino)3 (5 µM) for 24 h. Right panel: Quantification of the fluorescence. E–F Lipid ROS levels and LDH release in MDA-MB-231 cells after Fe(hino)3 (5 µM) treatment for 24 h following 2-h pretreatment with ROS scavengers, NAC (5 mM), or Trolox (200 µM). G Cell viability of MDA-MB-231 or 4T-1 cells after co-treatment with Fe(hino)3 (5 µM) and different concentrations of RSL3 for 24 h. H Representative electron micrographs of MDA-MB-231 cells treated with vehicle or Fe(hino)3 (5 µM) for 24 h. Arrows indicate the dead cells and the ruptured cell membrane, and triangles indicate the damaged mitochondria. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001

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