Fig. 8From: TSA-PACT: a method for tissue clearing and immunofluorescence staining on zebrafish brain with improved sensitivity, specificity and stabilityWorkflow of TSA-PACT. The brains are dissected out, embedded in hydrogel, eluted passively, fluorescence labeled for one or multiple rounds, matched refractive index and finally imaged using confocal, light sheet microscope, or other 3D imaging platformsBack to article page