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Fig. 7 | Cell & Bioscience

Fig. 7

From: TSA-PACT: a method for tissue clearing and immunofluorescence staining on zebrafish brain with improved sensitivity, specificity and stability

Fig. 7

Alterations of zebrafish brain proliferation pattern in a chronic hyperglycemia brain damage model. A Three-dimensional view of the whole brain with pHH3 staining of juvenile zebrafish. (Voxel size: 1.25 μm × 1.25 μm × 5 μm). B Three-dimensional view of the whole brain with pHH3 staining of adult zebrafish. (Voxel size: 1.25 μm × 1.25 μm × 5 μm). C Three-dimensional pHH3-positive cell counting analysis throughout the brain. The pHH3-positive cells were automatically identified and marked with pink spot. (Voxel size: 1.25 μm × 1.25 μm × 5 μm). D Three-dimensional pHH3-positive cell counting analysis for the telencephalon in chronic hyperglycemia (CHG) zebrafish and controls. CHG zebrafish were kept in fish water supplemented with 111 mM D-glucose over a period of 14 days. The pHH3-positive cells were automatically identified and marked with pink spot. (Voxel size: 1.25 μm × 1.25 μm × 5 μm). E Comparison of pHH3-positive cells in the telencephalon between CHG zebrafish and controls. The level of significance is calculated by unpaired Student’s t-test (mean ± S.E.M.; n = 5; ***p < 0.001)

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