Fig. 5

Zebrafish brain structural/cellular phenotyping with SMI312 and PV staining. A Three-dimensional view of the whole brain with SMI312 staining in juvenile zebrafish. The structure and distribution of major neurofilaments were displayed of brain-wide perspective. (Voxel size: 1.25 μm × 1.25 μm × 2 μm). B–E Images of supraoptic tract/lateral forebrain bundle, tract of the habenula commissure/habenula commissure, optic tract, ventral tectal fascicles, intertectal fascicles and commissures, cerebellar commissure and the ascending and descending pathway in juvenile zebrafish. (Voxel size: 1.25 μm × 1.25 μm × 2 μm for B and D; Voxel size: 0.624 μm × 0.624 μm × 2 μm for C and E). F Three-dimensional view of the whole brain with PV staining in juvenile zebrafish. (Voxel size: 1.25 μm × 1.25 μm × 5 μm). G–J Images of Purkinje cells, Purkinje cell layer and axonal tracts in the cerebellum, and PV-positive cells in olfactory bulb and optic tectum. Purkinje cells are with a big pear-shaped cell body and dendritic trees. They were gathered in Purkinje cell layer. (Voxel size: 0.312 μm × 0.312 μm × 1 μm for G; Voxel size: 1.25 μm × 1.25 μm × 2 μm for I and J)