Fig. 4
Zebrafish brain structural/cellular phenotyping with HuC/D staining. A Three-dimensional view of the whole brain with HuC/D staining in juvenile zebrafish. Several brain regions can be dissected, including olfactory bulb, telencephalon, optic tectum, cerebellum and medulla oblongata. (Voxel size: 1.25 μm × 1.25 μm × 5 μm). B–F Images of telencephalon, habenular nuclei and longitudinal torus, optic tectum, valvula of cerebellum with HuC/D staining in juvenile zebrafish. Neurons with large cell bodies were accumulated under the longitudinal torus. (Voxel size: 1.25 μm × 1.25 μm × 1 μm for C and E; Voxel size: 0.312 μm × 0.312 μm × 1 μm for D; Voxel size: 0.624 μm × 0.624 μm × 1 μm for F). G Three-dimensional view of the whole brain with HuC/D staining in adult zebrafish. Several brain regions can be recognized, including olfactory bulb, telencephalon, optic tectum, cerebellum and medulla oblongata. (Voxel size: 1.25 μm × 1.25 μm × 5 μm). H and I Images of optic tectum and neurons with large cell bodies with HuC/D staining in adult zebrafish. (Voxel size: 1.25 μm × 1.25 μm × 5 μm)