Fig. 1

Clarified zebrafish brain achieves optimal transparency and structural integrity. A Images of the brain of juvenile and adult zebrafish pre-clearing and post-clearing (85% glycerol or PACT). B and C Size changes after PACT clearing and the difference of transparency between glycerol clearing and PACT clearing in juvenile zebrafish brain (B) and adult zebrafish brain (C). Statistical significance in size changes and transparency difference was assessed by paired t test and unpaired t test, respectively. (mean ± S.E.M.; n = 5; ns, p > 0.05; *p < 0.05; **p < 0.01; and ***p < 0.001)