Fig. 7

Expression pattern of Nrg1 in the hypothalamus and thalamus. A Diagram of mouse brain sagittal section (top) and coronal section (bottom). The line in the sagittal section diagram indicates the position of the coronal section. The rectangle indicates the brain region shown in panel B. B Expression of tdTomato in the hypothalamus of 2-month-old Nrg1-reporting mice. Scale bar, 150 μm. C–E Fluorescent images of tdTomato, Gad67-GFP and NeuN in the VM C, ARC D and ME E from the rectangles in panel B. The solid arrows indicate Nrg1-positive cells which are GABAergic. The empty arrows indicate Nrg1-positive cells not expressing Gad67-GFP. Scale bars, 50 μm. The Nrg1-reporting mice were crossed with Gad67-GFP mice to visualize the expression of Nrg1 in GABAergic neurons. F Diagram of mouse brain sagittal section (left) and coronal section (right). The line in the sagittal section diagram indicates the position of the coronal section. The rectangle indicates the brain region shown in panel G. G Expression of tdTomato and Gad67-GFP in the SC of thalamus from 2-month-old Nrg1-reporting mice. Scale bar, 50 μm. H Fluorescent images of tdTomato, Gad67-GFP and NeuN from the rectangle in panel G. The solid arrows indicate GABAergic neurons expressing Nrg1. The empty arrow indicates GABAergic neurons not expressing Nrg1. Scale bar, 50 μm. DM: dorsomedial nucleus; VM: ventromedial nucleus; ARC: arcuate nucleus; ME: median eminence