Fig. 4

Expression pattern of Nrg1 in the striatum. A Diagram of mouse brain sagittal section (top) and coronal section (bottom). The line in the sagittal section diagram indicates the position of the coronal section. The rectangle indicates the brain region shown in panel B. B Expression of tdTomato in the striatum of 2-month-old Nrg1-reporting mice. Scale bar, 500 μm. C–D Fluorescent images of tdTomato and Gad67-GFP in the Tu C and AcbSH D from the rectangles in panel B. Arrows indicate Nrg1-positive cells which are GABAergic MSNs. Scale bar, 50 μm in panel C, 150 μm in panel D. The Nrg1-reporting mice were crossed with Gad67-GFP transgene mice to visualize the expression of Nrg1 in GABAergic MSNs. E Expression of tdTomato in axon fiber-like structures in the SNr. Scale bar, 300 μm. F–G Expression of tdTomato in the NAc F and SNr (G after injection of Cre-reporting AAV into the NAc of 2-month-old Nrg1^Cre/+ mice. Four weeks after AAV injection, the brain slices were collected and analyzed. Scale bar, 300 μm. H Double fluorescence in situ hybridization (dFISH) of Nrg1 and Drd1 mRNA in the striatum of WT mice. Scale bar, 300 μm. I The enlarged image from the rectangle in panel H. The solid arrows indicate Nrg1-positive cells expressing Drd1. The empty arrows indicate Nrg1-positive cells not expressing Drd1. Scale bar, 50 μm. CPu: caudate putamen; Den: dorsal endopiriform nucleus; aca: anterior commissure, anterior part; AcbC: accumbens nucleus, core; AcbSH: accumbens nucleus, shell; Tu: olfactory tubercle; SNr: substantia nigra, reticular part